Apple stem pitting virus (isolate PA66) (ASPV)

Taxonomy: Viruses; ssRNA viruses; ssRNA positive-strand viruses, no DNA stage; Tymovirales; Betaflexiviridae; Foveavirus; Apple stem pitting virus

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All


Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC_method)

Get csv file with sequences according given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 18 different methods for calculating isoelectric point
Protein with the lowest isoelectric point:
>sp|Q64962|RDRP_ASPVP RNA replication polyprotein
MALLSRTAAEEVIASFTSEEQSRISTQAVLALTNVEKDKHDLFNYALPELAKMRLFNSGIYLSPHSYRPHSHPVCKTLENNILFNILPSYLDNSFYLVSIKKNKVDFLKRRHPDLQMVETINRYISSIDKTRYGGFFHVSPSKISAKFKCDRRTGFEDDASLIDLIPGCMEGARKRFFFHDELHYWTKEA
LITFLDHVKPEVMLASIVFPPEILAGAKESLNPWCYTFRIVGKDLVFFPDGEQSEAYIQPVAGSYLLRTGKITTPSGDIFQLDLLKSSFSHHLISITKGEAIGQKMRFFNGFEAVAMKGLNPLRRKVESCLPISKNTILKIYRYLRTLKKPDLQSAMAKLSQVCKDPNGYEIKFFEEFSKLCLKCDTLNT
NMIPDMKRIVQGFFLKLFPNPISRNFKVVQQLHLDNFIETLEEFNFSINTESLSLNWKDDLEFVNLTFGDTDFNVEDSFAEAWGTKKDVVNITTVHHSPYLVSKFESYDHQFHSILSVKSISALTRIAKIVLSLYDPCVVEAFSESRVTNLAVNVIIAANLRACFAVTDLWRIFEGILLKECKRAQGKMR
KRFHFELGIRWFLFVDVSNQWFLPPCRDGLIARSVSFDQFIKGCQRDNSLHNGRMSLRQVLKGPKLQALFDVSELSIIHNVEMENAPEAGSTLDAGIKPTSSPLEVVPIENARCNLAPCKCDLNCFIQPADVNSLHGNLVFLDFIGGSKGRGASFYSRDLKGYSYTGFSHVSRGWPAFLDKFLSDNKIPL
NFYNQCLVQEYSTGHGLSMHKDDESIYDINHQVLTVNYSGDAIFCIECLGSGFEIPLSGPQMLLMPFGFQKEHRHGIKSPSKGRISLTFRLTKEGDSQVPIQEVVTICDHGDSDDRAALKALERRSHQSGGRPAVELEGHEREKVNSDSSDSAPVQEFLIQIDSSLLEYALKSLSGLSKNVVNCDMCLCN
SPWLKNEELRFSEALRDLAFAQGLIQLIDFLCLKVLRCAEVNRIISELPTHVFPLRGTMHIVDLDDESIRGDVKEGSFSGFRRWKVMSCSTDLIMLAFLKPKMTLGGELRSHEDECELSDLTEKLHGCSVILSRKFEPDLFHSFDVEADGNCFWHSVGPLIGVDGEYLKRILHDQAKKDGVKCPRLSKQL
EGNTWAEREAVAYFCSHYGIRLNVLYTREECTWIFKPHEVLKAATLICQDNHFKPCMPVNGCVIRAISSALNRREVDVLAVLGKPAHEDLFEEVAEGRGFSIFDLTRLFEIFSICGSVDTGGELIMVNENGRIPAEFSLEKEHLAHIPTLSRRKFSPIVSDLNRVSNSAMRFLAINGAEVDYRPSIDRAS
TLLDSFEIGATGVLCQGIKEAQKDLASKLIPELVHERKLIMILGTFGCGKSSLFKKFIEKSPGKAITFVSPRRSLAESINHDLGLARVGGKKTGKSKDLKNVRVKTFELFILHLDSIKEGHTVVIDEIQLFPPGYIDLIILGLKPNVNIIIAGDPCQSDYDCSSDRHIFAGSESDIMRILSGRSYKFNIL
SQRFRNPVFYGRLPCNLNKTRLTLDEEEYTLWDSIQEFSMMGRKDCPVVLVSSFEEKKIVAAHLGLKMKCITYGESTGLNFQKGAILVTYESALTSDRRWWTALSRFSHDIHFINGMGVTWDNAITHFVGKPLHKFFTKRACNDDIIDLLPGRPELIEGFQSQVGADEGVREAKLVGDPWLKTKIFLGQN
PDFEIEIADEVEAAEDWFKTHIPIMSLEAVRAQWVHKLISREDREFRIGDITTEQFTDDHSKNRGQELTNAAERYEAIYPRHKGTDTATFLMAVKKRLSFSSPAAEHAKLRRAKPFGKFLLDTFLKRVPLNSSHDEKMMQEAVHAFEEKKLSKSMATIENHSGRSCEDWPVDKALIFMKSQLCTKFDNRF
RSAKAGQTLACFQHSVLCRFAPYMRYIESKVTEVLPKNLYIHSGKNIDDLAAWVTTSKFNGVCTESDYEAFDASQDHFILAFELEVMKFLGLPSDLIADYTFIKTHLGSKLGSFAIMRFTGEASTFLFNTMANMLFTFLRYDLNGREAICFAGDDMCANSRLKVTNRFSNFLDKIKLKAKVQFTATPTFC
GWGLCEHGVFKKPDLVLERLQIARETRNLENCIDNYAIEVSCAYKMGENLNLYLTPQEVDAHYNCVRFIVQHNHLLKSNIRDLFKGESLPASS
Molecular weight: 247.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q64964|TGB2_ASPVP Movement protein TGB2
MPFAQPPDYSKSVFPIAVGIAVAVVLFTLTRSTLPQVGDNIHNLPHGGNYQDGTKRISYCGPRDSFPSSSLISSGTPMIIGIIIFLIFAIYVSEKWSRSGSRRCSCCVPGAPACTATVHE
Molecular weight: 12.85 kDa
Isoelectric point according different methods:





General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

3,010

70

2,183

602.0

67.3 kDa
Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

6.78

2.39

5.02

6.41

5.95

6.61

2.92

6.25

5.88

9.53

1.99

4.39

3.09

4.72

5.32

8.34

4.82

6.21

0.9

2.49
Note: For statistics only major isoforms were used (in this case 5 proteins)
For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI: proteome isoelectric point database. Nucleic Acids Res. 2016. doi: 10.1093/nar/gkw978       Contact: Lukasz P. Kozlowski