Proteome-pI - Proteome Isoelectric Point Database statistics

Escherichia coli (strain K12)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia; Escherichia coli

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 4,314 proteins (isoelectric point calculated using IPC_method)

Get csv file with sequences according given criteria:

* You can choose from 18 different methods for calculating isoelectric point

Protein with the lowest isoelectric point:

>sp|P25738|MSYB_ECOLI Acidic protein MsyB 

MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQDGDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGEFQLEPPLDTEEGRAAADEWDER

Molecular weight: 14.26 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>sp|P0A7P5|RL34_ECOLI 50S ribosomal protein L34 

MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTVSK

Molecular weight: 5.38 kDa
Isoelectric point according different methods:

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
4,306	8	4,314	1,356,101	14	2,358	314.9	34.9 kDa

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
9.52	1.16	5.15	5.76	3.89	7.37	2.27	6.01	4.41	10.67	2.82	3.95	4.44	4.43	5.51	5.8	5.4	7.07	1.53	2.85

Note: For statistics only major isoforms were used (in this case 4,306 proteins)
For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI: proteome isoelectric point database. Nucleic Acids Res. 2016. doi: 10.1093/nar/gkw978

Contact: Lukasz P. Kozlowski