Enterobacteria phage Phieco32 (Escherichia coli phage phi32)

Taxonomy: Viruses; dsDNA viruses, no RNA stage; Caudovirales; Podoviridae; Phieco32likevirus

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All


Virtual 2D-PAGE plot for 128 proteins (isoelectric point calculated using IPC_method)

Get csv file with sequences according given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 18 different methods for calculating isoelectric point

Protein with the lowest isoelectric point:
>tr|B0FIR5|B0FIR5_BPE32 Putative uncharacterized protein
MSCFGGGEIDDDYDMESNGTCPDCGCETFDGVSVYPACSYSPVICKTCGDQPCDQSC
Molecular weight: 6.01 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|B0FIM0|B0FIM0_BPE32 Putative uncharacterized protein
MADHVGKRDYAQERRTAIARGETGVGSKSGDAQRHRARRIVEKRDGKAAVRGKDVGHKRAVKSGGSNASSNLRVESVSSNRSNGGKSGDRAGKAAGARKGALSSS
Molecular weight: 10.91 kDa
Isoelectric point according different methods:






General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

128

0

128

23,545

35

1,473

183.9

20.6 kDa

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

7.82

1.23

6.15

6.94

3.85

7.25

1.74

5.69

6.28

7.39

2.84

5.67

4.17

3.63

5.14

6.8

5.73

6.79

1.46

3.44

Note: For statistics only major isoforms were used (in this case 128 proteins)
For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI: proteome isoelectric point database. Nucleic Acids Res. 2016. doi: 10.1093/nar/gkw978       Contact: Lukasz P. Kozlowski